Are You Fresher/Experience Candidate , Are you interested in Pharma Industrial Jobs ? Are you interested in Pharma Quality Control Jobs ? Then we are suggest various interview Question and Answers to prepare well for the interview. Then your solution would be in PharmaFreshJobs, because here In PharmaFreshJob we have provided you the complete detailed interview questions covering all Type of nooks and corner topics in Pharma Quality Control. PharmaFreshJob it is the one of the Platforms to provide all type of Industrial & Pharma Jobs & Different Type Of Tips.
Pharma Quality Control Jobs Some Imp Interview Question and Answers
Q.1. Explain The Principle Of Ultraviolet Spectroscopy?
Answer : UV spectroscopy uses light in the UV part of electromagnetic spectrum. UV absorption spectra arises in which molecule or atoms outer electrons absorb energy, undergoes transition from lower energy level to higher energy level. For each molecule, absorbance at wavelength is specific.
Q.2. Explain About Beer Lamberts Law?
Answer : It states that the intensity of monochromatic light absorbed by a substance dissolved in a fully transmitting solvent is directly proportional to the substance concentration and the path length of the light through the solution.
Q.3. Explain The Infrared Spectroscopy Principle?
Answer : When a molecule absorbs the Infrared radiation, it vibrates and gives rise to packed Infrared(IR) absorption spectrum. This IR spectrum is specific for every different molecule absorbing the IR radiation, useful for its identification.
Q.4. Define pH? What is the pH of Blood?
Answer : pH -Negative logarithm of hydrogen ion concentration. Blood pH-7.35 to 7.45.
Q.5. Expand Full form of LCMS, HPLC,UPLC, TLC And GC?
Answer : LCMS- Liquid Chromatography
HPLC- High Performance Liquid Chromatography,
UPLC- Ultra High Performance Liquid Chromatography,
TLC- Thin Layer Chomatography,
GC- Gas Chromatography.
Q.5. What is the HPLC Principle?
Answer : It is a technique used for separating the mixture of components into individual components based on adsorption, partition, ion exchange and size exclusion principles. Stationary phase and mobile phase used in it. HPLC used for identification, quantification and purification of components form a mixture.
Q.6. Explain HPLC Instrumentation?
Answer : It involves solvent system, pump, Sample injector, HPLC columns, Detectors and Recorder. Firstly, solvent(mobile phase) is degassed for eliminating the bubbles. It is passed through the pump with a uniform pressure. The liquid sample is injected into the mobile phase flow stream. It passes through the stationary phase identified by the detectors and recorded.
Q.6. In Reverse Phase HPLC, Which type of Stationary Phase is used and give Example?
Answer : Non polar stationary phase used
Ex: Silica gel C-18
Q.7. What are the Detectors used in HPLC?
Answer : UV detector, IR detector, Fluorescence detector, Mass spectroscopy, LC MS etc.
Q.8. How to Calculate Retention Factor in Paper Chromatography?
Answer : Rf = Distance travelled by solute/ Distance travelled by solvent.
Q.9. Define Molarity?
Answer : Number of moles of solute per litre solution. Denoted with “M”
Q.10. Define Molality?
Answer : Number of moles of solute per kilogram solvent. Denoted with “m”
Q.11. Define Normality?
Answer : Number of Number of moles equivalent per litre solution.
Q.12. Difference Between Humidity And Relative Humidity?
Answer : Humidity – Measure of amount of water vapour present in the atmosphere.
Relative humidity- Water vapour amount exists in air expressed as a percentage of the amount needed for saturation at the same temperature.
Q.13. What Is The Difference Between Qualitative And Quantitative Analysis?
Answer : Qualitative analysis involves identification of the compound or chemical based on their chemical(absorption, emission )or physical properties(e.g Melting point, boiling point).
Quantitative analysis involves estimation or determination of concentration or amount of the chemical compounds or components.
Q.14. What Is The Ultraviolet(UV) And Visible Spectroscopy Range?
Answer : UV spectroscopy range 200-400 nm, Visible spectroscopy range 400 nm to 800nm.
Q.15. What Is The Use Of UV Spectroscopy?
Answer : Spectroscopy used for detecting the functional groups, impurities. Qualitative and quantitative analysis can be done.
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